Journal: Scientific Reports

Article Title: Opto-electrical bimodal recording of neural activity in awake head-restrained mice

doi: 10.1038/s41598-021-04365-7

Figure Lengend Snippet: Bimodal recording of neuronal calcium signalling and LFP in CA1 using a GRIN lens and a silicon probe. ( a ) Position of a GRIN lens and a silicon probe for bimodal recordings in the CA1 area. ( b ) Confirmation of the placement of the GRIN lens and the silicon probe. Top: A frame of fluorescence image showing the existence of silicon probe shanks under the GRIN lens (Arrowheads). Bottom: An image of a cresyl violet-stained brain section showing the location of the four shanks of the silicon probe (white dotted circles). Orange dashed line indicates the estimated position of the GRIN lens. ( c ) Left: A ΔF/F image showing Individual neurons expressing GCaMP6s. Right: Example frames indicating changes in fluorescence during a calcium event for each of four neurons. Colors correspond to colors of dashed circles in the left image. ( d ) An example of temporally matched traces of LFP and neuronal calcium signals. Raw and band pass-filtered traces (150–250 Hz) are presented in black and blue lines, respectively. The band pass-filtered traces show multiple episodes of sharp wave-associated ripples. Calcium signals from different neurons are presented in different colours. ( e ) Another example temporally matched traces while the mouse runs. We observed clear theta oscillations (8–12 Hz). Data in this figure were recorded 32 days after implantation.

Article Snippet: We used viral vectors expressing GCaMP6f under the control of the regulatory element from the GFAP gene (AAV5/pZac2.1 gfaABC1D-cyto-GCaMP6f, prepared by Addgene, a gift from Dr. Baljit Khakh, Addgene plasmid # 52925) for Fig. and GCAMP6s under the control of the regulatory element from the CaMKIIα gene [prepared as previously described (Kitanishi et al., 2015)] for Fig. .

Techniques: Fluorescence, Staining, Expressing